Table 1.
Analysis of mouse NR gene expression in 3T3-L1 adipocytes treated with NDP-MSH
| Detector | B value | t value | P value | Adjusted P value (FDR) | Log10 RQ | Linear RQ | Ct status |
|---|---|---|---|---|---|---|---|
| Ar-Mm00442688_m1 | −7.22399868158938 | 0.73208 | 0.49669 | 0.73078 | −0.064420419072092 | 0.86214 | Valid |
| Esr1-Mm00433149_m1 | −7.24969871166557 | 0.69895 | 0.51547 | 0.73078 | −0.254771719663615 | 0.55620 | No detection |
| Esr2-Mm00599819_m1 | −7.48900909552998 | 0.25705 | 0.80729 | 0.88068 | −0.142688217944727 | 0.71997 | Target not detected |
| Esrra-Mm00433143_m1 | −7.42996 | −0.408922825843263 | 0.69937 | 0.83127 | 0.04114 | 1.09936 | Valid |
| Esrrb-Mm00442411_m1 | −7.1667471587223 | 0.80187 | 0.45870 | 0.73078 | −0.382809811152694 | 0.41418 | No detection |
| Esrrg-Mm00516267_m1 | −6.80068 | −1.17387867497104 | 0.29284 | 0.56322 | 0.97273 | 9.39136 | Calibrator not detected |
| Hnf4a-Mm00433964_m1 | −7.09361679187359 | −0.884738688556906 | 0.41645 | 0.73078 | 0.17359 | 1.49140 | Valid |
| Hnf4g-Mm00443563_m1 | −4.94556729942662 | −2.61847886950877 | 0.04676 | 0.17264 | 0.15112 | 1.41618 | No detection |
| Dax-Mm00431729_m1 | −4.94557 | −2.61847886950877 | 0.04676 | 0.17264 | 0.15112 | 1.41618 | No detection |
| Shp-Mm00442278_m1 | −7.22980712143738 | 0.72470 | 0.50083 | 0.73078 | −0.280660299290717 | 0.52401 | No detection |
| Rev-erba-Mm00520708_m1 | −4.03480 | −3.32846032259584 | 0.02052 | 0.16418 | 0.16818 | 1.47291 | Valid |
| Rev-erb-Mm00441730_m1 | −7.38390470987611 | −0.497475417556279 | 0.63977 | 0.80813 | 0.04144 | 1.10012 | Valid |
| Lxrb-Mm00437262_m1 | −7.44585 | 0.37389 | 0.72368 | 0.83127 | −0.0349194794970221 | 0.92274 | Valid |
| Lxra-Mm00443454_m1 | −7.44176015428787 | −0.383196999385467 | 0.71718 | 0.83127 | 0.02669 | 1.06339 | Valid |
| Fxra-Mm00436419_m1 | −7.19653 | 0.76620 | 0.47784 | 0.73078 | −0.307451968904812 | 0.49266 | No detection |
| Fxrb-Mm01308716_m1 | −4.94556729942662 | −2.61847886950877 | 0.04676 | 0.17264 | 0.15112 | 1.41618 | No detection |
| Pxr-Mm00803092_m1 | −6.20661469567789 | −1.66706424293002 | 0.15583 | 0.41926 | 0.62303 | 4.19790 | No detection |
| Car-Mm00437986_m1 | −6.15168390378067 | 1.70975 | 0.14746 | 0.41926 | −0.566337765175836 | 0.27143 | No detection |
| Tr2-Mm00449123_m1 | −7.52801490090376 | 0.01864 | 0.98584 | 0.99802 | −0.00150514997832014 | 0.99654 | Valid |
| Tlx-Mm00455855_m1 | −4.94556729942662 | −2.61847886950877 | 0.04676 | 0.17264 | 0.15112 | 1.41618 | No detection |
| Pnr-Mm00443299_m1 | −4.94556729942662 | −2.61847886950877 | 0.04676 | 0.17264 | 0.15112 | 1.41618 | No detection |
| COUP-TF1-Mm00657937_m1 | −2.27214 | −4.90398041511805 | 0.00435 | 0.04173 | 0.17520 | 1.49692 | Valid |
| COUP-TF2-Mm00772789_m1 | −7.33692467922958 | 0.57502 | 0.58996 | 0.76536 | −0.0309057462215019 | 0.93131 | Valid |
| Ear2-Mm00438762_m1 | −7.51584 | 0.14413 | 0.89098 | 0.95038 | −0.00872986987425464 | 0.98010 | Valid |
| Gr-Mm00433832_m1 | −6.71352460251767 | −1.25164027630488 | 0.26558 | 0.56322 | 0.04154 | 1.10038 | Valid |
| Mr-Mm01241595_m1 | −6.68485477267374 | −1.27665610100014 | 0.25731 | 0.56322 | 0.16296 | 1.45532 | Valid |
| Nur77-Mm00439358_m1 | 8.21414 | −43.5475587664681 | 1.06825E-07 | 0.00001 | 1.50224 | 31.78630 | Valid |
| Nurr1-Mm00443056_m1 | 1.72159 | −10.4452421876562 | 0.00013 | 0.00192 | 1.88806 | 77.27876 | Valid |
| Nor-1-Mm00450074_m1 | 6.90613 | −29.8588159527 | 7.12918E-07 | 0.00002 | 2.00948 | 102.20581 | Valid |
| Sf-1-Mm00446826_m1 | −7.52050115581881 | −0.113763265382575 | 0.91381 | 0.95354 | 0.08208 | 1.20804 | Valid |
| Lrh-1-Mm00446088_m1 | −4.94556729942662 | −2.61847886950877 | 0.04676 | 0.17264 | 0.15112 | 1.41618 | No detection |
| Gcnf-Mm00599848_m1 | −7.18346812239051 | −0.78199782533274 | 0.46930 | 0.73078 | 0.09412 | 1.24200 | Valid |
| Pgr-Mm00435625_m1 | −6.3847189015981 | −1.52628688212191 | 0.18693 | 0.46694 | 0.77124 | 5.90526 | No detection |
| Ppara-Mm00440939_m1 | −7.29437689329319 | 0.63803 | 0.55127 | 0.73503 | −0.122418864903352 | 0.75436 | Valid |
| Ppard-Mm00803186_g1 | −6.75885243212593 | −1.2115411614176 | 0.27934 | 0.56322 | 0.04475 | 1.10854 | Valid |
| Pparg-Mm00440945_m1 | −5.18066 | −2.44140307841398 | 0.05809 | 0.19916 | 0.07817 | 1.19720 | Valid |
| Rara-Mm00436264_m1 | −6.21541383789805 | 1.66020 | 0.15722 | 0.41926 | −0.0782677988726348 | 0.83509 | Valid |
| Rarb-Mm01319676_m1 | −6.80219 | 1.17251 | 0.29334 | 0.56322 | −0.518373652533376 | 0.30313 | Valid |
| Rarg-Mm00441083_m1 | −7.27150349322754 | −0.669801501139277 | 0.53240 | 0.73078 | 0.09111 | 1.23342 | Valid |
| Rora-Mm00443103_m1 | −5.85406 | −1.93695242609036 | 0.10996 | 0.35188 | 0.09141 | 1.23428 | Valid |
| Rorb-Mm00524993_m1 | −7.46572615251607 | 0.32514 | 0.75812 | 0.84628 | −0.145999547897031 | 0.71450 | Calibrator not detected |
| Rorc-Mm00441139_m1 | −7.27208 | −0.6690192569434 | 0.53286 | 0.73078 | 0.02639 | 1.06265 | Valid |
| Rxra-Mm00441182_m1 | −7.52821835574711 | 0.00261 | 0.99802 | 0.99802 | −0.00010034333188812 | 0.99977 | Valid |
| Rxrb-Mm00441193_m1 | −6.42324698260015 | −1.49524809558933 | 0.19456 | 0.46694 | 0.05780 | 1.14235 | Valid |
| Rxrg-Mm00436410_m1 | −4.94556729942662 | −2.61847886950877 | 0.04676 | 0.17264 | 0.15112 | 1.41618 | No detection |
| Thra-Mm00617505_m1 | −7.24746389971597 | 0.70188 | 0.51379 | 0.73078 | −0.0276947596010864 | 0.93822 | Valid |
| Thrb-Mm00437044_m1 | −7.44812283948188 | 0.36863 | 0.72736 | 0.83127 | −0.0499709792802206 | 0.89131 | Valid |
| Vdr-Mm00437297_m1 | 1.50169 | −10.0309216281866 | 0.00016 | 0.00192 | 0.59734 | 3.95680 | Valid |
Table displays the P values, and adjusted P values of target genes before and after the application of FDR, respectively. Please note that genes with adjusted P values less than 0.05, after FDR, are differentially expressed in a significant manner. However, some of these genes have a negative B score because Bayes analysis stringently considers a proportion of differentially expressed genes with P < 0.01, and providesinformation about their ranking (Integromics Technical Support, Joaquin Panadero, personal communication). Analysis also includes t, the empirical Bayes moderated t statistic (a variant t test), an empirically moderated estimate of standard error. The relative quantification (RQ) linear and log10 denotes the calculated fold differences [between the target (NDP-MSH treatment) and the calibrator/ reference (vehicle treatment) in a linear and log scale, respectively], normalized against the mean of the Genorm selected controls, 18S rRNA, Gapdh, and GusB. Bold indicates genes differentially expressed in a significant manner.