Table 4.
Analysis of mouse metabolic gene expression in 3T3-L1 adipocytes treated with 8-Br cAMP
| Detector | B value | t value | P value | Adjusted P value (FDR) | Linear RQ | Log10 RQ | Ct status |
|---|---|---|---|---|---|---|---|
| Abca1-Mm00442646_m1 | −6.47203270416448 | −0.983136965483622 | 0.35911 | 0.58168 | 1.05340 | 0.02260 | Valid |
| Abcg1-Mm00437390_m1 | −6.94866431433544 | −0.255521028063199 | 0.80585 | 0.89376 | 1.04779 | 0.02028 | Valid |
| Acadm-Mm00431611_m1 | −6.95730730202809 | −0.223512091415063 | 0.82968 | 0.90376 | 1.02976 | 0.01274 | Valid |
| Acsl4-Mm00490331_m1 | −2.99892919267858 | −3.51728759197849 | 0.01018 | 0.05644 | 2.41953 | 0.38373 | Valid |
| Akt2-Mm00545827_m1 | −5.99484250094875 | −1.41007012605418 | 0.20244 | 0.41631 | 1.23313 | 0.09101 | Valid |
| Apoe-Mm00437573_m1 | −5.9960202210425 | −1.40911893801009 | 0.20271 | 0.41631 | 1.21792 | 0.08562 | Valid |
| Cav1-Mm00483057_m1 | −6.4253521928257 | −1.03004427920842 | 0.33811 | 0.57291 | 31.77646 | 1.50211 | Valid |
| Cav3-Mm01182632_m1 | −6.58672998372099 | −0.859854864267424 | 0.41905 | 0.60862 | 40.62194 | 1.60876 | No detection |
| Cd36-Mm00432403_m1 | −6.00486578483379 | −1.40196357076678 | 0.20474 | 0.41631 | 1.12745 | 0.05210 | Valid |
| Cebpa-Mm00514283_s1 | −5.30471603316879 | −1.92631942576746 | 0.09652 | 0.26762 | 1.39442 | 0.14439 | Valid |
| Cebpb-Mm00843434_s1 | 0.77510 | −6.75103121847619 | 0.00030 | 0.00603 | 2.02460 | 0.30634 | Valid |
| Cebpd-Mm00786711_s1 | −3.65958645529927 | −3.05428013704486 | 0.01906 | 0.08200 | 1.56833 | 0.19544 | Valid |
| Cox5a-Mm00432638_m1 | −5.22622058282471 | − | 0.08903 | 0.26023 | 1.28389 | 0.10853 | Valid |
| Cpt1b-Mm00487200_m1 | −6.12619850864466 | 1.30163 | 0.23527 | 0.46294 | 0.10928 | −0.96145793125541 | Target not detected |
| Cpt2-Mm00487202_m1 | −6.51347921922502 | −0.940037726418302 | 0.37928 | 0.58586 | 1.09284 | 0.03856 | Valid |
| Cs-Mm00466043_m1 | −6.22855102872073 | 1.21326 | 0.26536 | 0.50584 | 0.91042 | −0.0407596072971188 | Valid |
| Fabp4-Mm00445880_m1 | −4.4826065167765 | −2.49285759987093 | 0.04228 | 0.13936 | 1.35951 | 0.13338 | Valid |
| Fasn-Mm00662319_m1 | −6.9701887124759 | −0.164829827780038 | 0.87385 | 0.93518 | 1.01482 | 0.00639 | Valid |
| Foxo1-Mm00490672_m1 | −5.68269700553692 | 1.65195 | 0.14364 | 0.35048 | 0.83745 | −0.0770421500450454 | Valid |
| Gys1-Mm00472712_m1 | −5.65091641789539 | −1.67564891864883 | 0.13883 | 0.35048 | 1.15577 | 0.06287 | Valid |
| Hif1a-Mm00468875_m1 | −0.529115798487438 | −5.48088372472817 | 0.00101 | 0.01025 | 1.59144 | 0.20179 | Valid |
| Hk2-Mm00443385_m1 | −0.132955099353836 | −5.84542007115945 | 0.00070 | 0.00849 | 2.17440 | 0.33734 | Valid |
| Il6-Mm00446190_m1 | −6.52307398754988 | −0.929844051263675 | 0.38417 | 0.58586 | 4.11340 | 0.61420 | Valid |
| Insr-Mm00439693_m1 | −5.7412347069987 | −1.60791890248564 | 0.15300 | 0.35896 | 1.16573 | 0.06660 | Valid |
| Irs1-Mm00439720_s1 | −5.60423389368891 | −1.71021227014467 | 0.13208 | 0.35030 | 1.14670 | 0.05945 | Valid |
| Lep-Mm00434759_m1 | −6.47892150309319 | 0.97607 | 0.36236 | 0.58168 | 0.88855 | −0.0513194155804653 | Valid |
| Lepr-Mm00440181_m1 | −5.23233463895439 | −1.97738797918858 | 0.08959 | 0.26023 | 2.38960 | 0.37833 | No detection |
| Lipe-Mm00495359_m1 | −3.71816685794912 | −3.01393427787688 | 0.02016 | 0.08200 | 1.31521 | 0.11900 | Valid |
| Lpin1-Mm00550511_m1 | −1.80312364585439 | −4.41121255918593 | 0.00331 | 0.02526 | 1.78729 | 0.25220 | Valid |
| Lpl-Mm00434764_m1 | −4.50941649424105 | −2.47464242692057 | 0.04341 | 0.13936 | 1.22113 | 0.08676 | Valid |
| Ndufa5-Mm00471676_g1 | −4.04833801951533 | −2.78799300323631 | 0.02770 | 0.10243 | 1.23020 | 0.08997 | Valid |
| Ndufb5-Mm00452592_m1 | −6.55152761517444 | −0.899090279456486 | 0.39922 | 0.59396 | 1.11127 | 0.04582 | Valid |
| Npy-Mm00445771_m1 | −6.98517697252056 | −0.0273625061673953 | 0.97895 | 0.98896 | 1.11408 | 0.04691 | No detection |
| Npy2r-Mm01956783_s1 | −6.60884715447981 | 0.83445 | 0.43227 | 0.60866 | 0.03293 | −1.48243364236616 | Target not detected |
| Pck1-Mm00440636_m1 | 1.79158 | −7.90330823645043 | 0.00011 | 0.00603 | 3.73391 | 0.57216 | Valid |
| Pdk1-Mm00554306_m1 | −6.71401786122737 | −0.70357736013987 | 0.50499 | 0.66966 | 1.05464 | 0.02311 | Valid |
| Pdk2-Mm00446681_m1 | −2.68222632945434 | −3.74592162059326 | 0.00755 | 0.04996 | 1.39402 | 0.14427 | Valid |
| Pdk3-Mm00455220_m1 | −6.85057901261473 | −0.491669112042786 | 0.63837 | 0.81127 | 1.03864 | 0.01647 | Valid |
| Pdk4-Mm00443325_m1 | −2.76858195305639 | −3.6830748059515 | 0.00819 | 0.04996 | 1.45149 | 0.16182 | Valid |
| Pfkm-Mm00445461_m1 | −6.31725965485071 | −1.13319036867258 | 0.29538 | 0.52994 | 11.56542 | 1.06316 | Valid |
| Pnpla2-Mm00503040_m1 | −6.9842209070256 | −0.0493042992031737 | 0.96209 | 0.98896 | 1.01255 | 0.00541 | Valid |
| Ppargc1a-Mm00447183_m1 | 0.81440 | −6.79266553187554 | 0.00029 | 0.00603 | 1.71710 | 0.23480 | Valid |
| Ppargc1b-Mm00504720_m1 | 0.29347 | −6.25776701118953 | 0.00047 | 0.00711 | 2.31327 | 0.36423 | Valid |
| Ptgs2-Mm00478374_m1 | −6.887285384296 | −0.418544035436358 | 0.68841 | 0.83964 | 2.25203 | 0.35257 | Valid |
| Pygm-Mm00478582_m1 | −6.78200615886998 | −0.606462579597974 | 0.56383 | 0.73178 | 1.12981 | 0.05300 | Valid |
| Rab18-Mm00441057_m1 | −6.29048302233768 | −1.15774464650995 | 0.28588 | 0.52845 | 8.89780 | 0.94928 | Valid |
| Retn-Mm00445641_m1 | −3.3695485144151 | −3.25555636819725 | 0.01446 | 0.06785 | 1.38660 | 0.14195 | Valid |
| Rock1-Mm00485745_m1 | −6.98548553682099 | −0.0143483063672784 | 0.98896 | 0.98896 | 1.00123 | 0.00053 | Valid |
| Rock2-Mm00485761_m1 | −3.26541588310136 | −3.32853000857313 | 0.01310 | 0.06658 | 1.32339 | 0.12169 | Valid |
| Scd1-Mm00772290_m1 | −6.90752779559935 | −0.372484398662658 | 0.72083 | 0.83964 | 1.03855 | 0.01643 | Valid |
| Scd2-Mm00485951_g1 | −6.87819738040745 | −0.437720434408795 | 0.67511 | 0.83964 | 1.04912 | 0.02082 | Valid |
| Slc2a2-Mm00446224_m1 | −6.93605143481426 | −0.821666151576023 | 0.43903 | 0.60866 | 21.59963 | 1.33445 | Calibrator not detected |
| Slc2a4-Mm00436615_m1 | 0.29619 | 0.77590 | 0.87647 | 0.96984 | −0.0133017511643106 | Valid | |
| Slc2a8-Mm00444634_m1 | −5.93755681705318 | −1.45593067223172 | 0.18983 | 0.41631 | 1.11031 | 0.04544 | Valid |
| Socs3-Mm00545913_s1 | −6.98087310901216 | 0.09124 | 0.92992 | 0.97802 | 0.99436 | −0.00245751920288478 | Valid |
| Srebf1-Mm00550338_m1 | −6.90082006732795 | 0.38833 | 0.70961 | 0.83964 | 0.95199 | −0.0213659988995519 | Valid |
| Stat3-Mm00456961_m1 | −6.91250683871165 | −0.360294547303217 | 0.72952 | 0.83964 | 1.09045 | 0.03761 | Valid |
| Stat5b-Mm00839889_m1 | −6.38906309223622 | 1.06546 | 0.32291 | 0.56279 | 0.89213 | −0.0495705205996298 | Valid |
| Ucp1-Mm00494069_m1 | −6.71353806416891 | 0.70422 | 0.50461 | 0.66966 | 0.02429 | −1.61448796705966 | No detection |
| Ucp2-Mm00495907_g1 | −1.30242634524268 | −4.81470379568001 | 0.00208 | 0.01809 | 1.49891 | 0.17577 | Valid |
| Ucp3-Mm00494074_m1 | −4.0794328594065 | −2.76681380479372 | 0.02854 | 0.10243 | 1.29796 | 0.11326 | Valid |
Table displays the P values, and adjusted P values of target genes, before and after the application of FDR. Relative quantification (RQ) linear and log10 were normalized against the mean of the Genorm selected controls 18S rRNA, Gapdh, and 36b4. Statistical analysis as detailed in Table 1. Bold indicates genes differentially expressed in a significant manner.