Fig. 3.

Ternary complex formation by ALS and D440N-ALS. Culture media from HepG2 cells infected with adenoviruses, containing 3 pmol of either recombinant wild-type ALS (triangles) or D440N-ALS (circles) estimated by immunoblot, were preincubated with 1 pmol of IGFBP-3 and 100,000 cpm of [¹²⁵I]IGF-I before fractionation on a Superose-12 column. The amount of radioactivity was determined in each fraction. As a control, a similar reaction was performed with an equal volume of culture media from cells infected with empty vector virus (squares).