Fig. 1.

GnRH-II acts in an autocrine manner to enhance ovarian cancer cell invasion. OVCAR-3 (A) and CaOV-3 (B) cells were treated with 100 nm GnRH-II siRNA (si-GnRH-II) or 100 nm control siRNA (si-Ctrl) for 24 h. In both cases, the mRNA levels of GnRH-II and GnRHR were measured by RT-qPCR and expressed as mean ± sem of three independent experiments. *, P < 0.05 compared with control siRNA (si-Ctrl). C and D, The cells were then seeded into Matrigel-coated transwells and cultured for 48 h in the presence of 10 nm GnRH-II. Noninvading cells were removed from the upper side of the filter, and nuclei of invading cells were stained with Hoechst 33258. Upper panels show representative photomicrographs of cells attached to the lower membrane of the transwells in the invasion assays, while lower panels show the quantitative results of these assays, with results expressed as mean ± sem of three independent experiments. *, P < 0.05 compared with control siRNA (si-Ctrl).