Fig. 5.

The laminin binding domain of LRP plays an important role in ovarian cancer cell invasion. A, CaOV-3 cells were stably transfected with wild-type LRP (LRP) or two different mutated LRP expression vectors (i.e., Mut A, Mut B). The relative LRP levels in these cells, and cells expressing eGFP as a control (Ctrl), were compared by Western blotting (upper panel), and invasion assays using these cells were performed for 48 h (lower panel). B, In parallel experiments, these stably transfected cells were also subjected to adhesion assays for 8 h. In both A and B, results are expressed as mean ± sem of three independent experiments. *, P < 0.05 compared with the control cells (Ctrl).