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. 2017 Apr 28;73(Pt 5):300–304. doi: 10.1107/S2053230X17006173

Table 2. Crystallization.

Method Microbatch under oil
Plate type 72-well microbatch plates
Temperature (K) 295
Protein concentration (mg ml−1) 7
Buffer composition of protein solution 30 mM Tris–HCl pH 7.4, 300 mM NaCl, 10 mM imidazole, 10%(v/v) glycerol
Composition of reservoir solution 6%(v/v) Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25%(w/v) polyethylene glycol 4000
Volume and ratio of drop 4 µl drop: 2 µl protein solution + 2 µl crystallization condition