Fig. 8.

SFK monobodies activate autoinhibited recombinant Src and Hck. (a) Schematic representation of the in vitro kinase assay setup, in which recombinant Src or Hck or preincubated with the SFK negative regulatory kinase Csk and/or recombinant monobodies before assaying the phosphorylation of a SFK substrate peptide with a continuous spectrophotometric assay. (b and c) In vitro kinase activity of Src and Hck was measured in the absence or presence of Csk and set to 1.0. Relative changes in kinase activity are shown at the indicated concentrations of (b) Mb(Yes_1) or (c) Mb(Lck_3). Each data point corresponds to the average of three repeats +/− SD. Control experiments are shown in SI Fig. 10.