Table 3.
Inhibition of CYP11B enzymes by fadrozole in comparison with other steroidogenic P450s
| Cytochrome P450 | Kd, nm | Ki(app), nm |
|---|---|---|
| CYP11B1 (steroid 11β-hydroxylase) | 9 ± 1 | 14.8 ± 2 |
| CYP11B2 (aldosterone synthase) | 370 ± 53 | 354 ± 39 |
| CYP19A1 (aromatase) | 16 ± 2 | 45 ± 7 |
| CYP17A1 (steroid 17α-hydroxylase/17,20-lyase) | 162 ± 29 | NM |
| CYP21A2 (steroid 21-hydroxylase) | ND | NM |
The apparent dissociation constant Kd was determined by differential spectral titration using low-spin P450s. NM, Not measured; ND, not detected.