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. 2012 Dec 28;27(2):192–202. doi: 10.1210/me.2012-1247

Fig. 2.

Fig. 2.

Glucocorticoids selectively modulate the expression of decidual marker genes. A, RTQ-PCR analysis of 11βHSD1 transcript levels in HESCs treated with 8-bromo-cAMP with or without P4 or DEX for 2, 4, and 8 d. The results show the fold change (mean ± sem) in 11βHSD1 transcript levels relative to vehicle control of four independent primary cultures. B, Western blot analysis of GR, MR, 11βHSD1, and PR proteins in whole-cell lysates obtained from primary HESCs treated with a combination of 8-bromo-cAMP with or without P4 or DEX for 4 d. β-Actin served as a loading control. C and D, Fold change (mean ± sem) in PRL and IGFBP1 mRNA levels, respectively, relative to vehicle control of four independent primary cultures. Different letters above the error bars indicate that those groups are significantly different from each other at P < 0.05.