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. 2012 Dec 28;27(2):192–202. doi: 10.1210/me.2012-1247

Fig. 5.

Fig. 5.

GR signaling plays a role in chromatin remodeling in decidualizing HESCs. A, ZNF486 transcript levels were measured in four primary cultures treated 8-bromo-cAMP, P4, and E for the indicated time points (left panel) as well as in cultures decidualized for 4 d after GR or MR knockdown (right panel). The results show mean fold change (±sem) relative to levels in undifferentiated cells (dotted lines). *, P < 0.05. B, Western blot analysis of H3K9me3 expression in total protein lysates from primary cultures treated 8-bromo-cAMP, P4, and E for the indicated time points (left panel) as well as from cultures decidualized for 4 d upon GR or MR knockdown (right panel). β-Actin served as a loading control.