Fig. 6.

MR-dependent induction of DHRS3 in decidualizing HESCs is associated with dynamic changes in cytoplasmic lipid droplets. DHRS3 expression was examined in parallel primary cultures treated with 8-bromo-cAMP, P4, and E for the indicated time points as well as after GR or MR siRNA knockdown by RTQ-PCR (A) or Western blot analysis (B). DHRS3 expression was analyzed in four independent primary cultures. The left panel shows percentage change (±sem) in mRNA expression relative to levels in undifferentiated cells (dotted lines). C, Confocal micrographs showing lipid droplets stained with BODIPY (green) and nuclei stained with 4,6-diamidino-2-phenylindole (DAPI) (blue) in HESCs treated with 8-bromo-cAMP, P4, and E for the indicated time points. D, Representative confocal images of HESCs decidualized with 8-bromo-cAMP and P4 in the presence or absence of aldosterone or RU26752 for 8 d. Scale bar, 10 μm.