Figure 2.

ATP-hydrolysis activity assay of DENV-2 and DENV-4 NS3 proteins. (a) An elution diagram of purified DENV-4 NS2B₁₈NS3 using a Superdex 200 HR 10/300 column. The fractions from the shaded region were pooled and concentrated. Inset: the SDS gel shows a highly pure protein, which is suitable for SAXS studies. (b) ATP-hydrolysis activity data reveal that the recombinant NS2B₁₈NS3 and NS3 helicase domains from DENV-2 and DENV-4 are active. (c) ATP-hydrolysis activity bar diagram for DENV-2 NS2B₁₈NS3 and its helicase domain in the absence or presence of the effectors resveratrol, quercetin and NBD-Cl. In the presence of resveratrol and quercetin ATP hydrolysis was inhibited in both DENV-2 NS2B₁₈NS3 and the helicase domain, whereas in the presence of NBD-Cl ATP hydrolysis was enhanced in both NS2B₁₈NS3 and the helicase domain.