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. Author manuscript; available in PMC: 2017 Nov 7.
Published in final edited form as: Integr Biol (Camb). 2016 Nov 7;8(11):1133–1144. doi: 10.1039/c6ib00139d

Figure 2. Time course measurements of cell morphology and biomarker expression with Snail-1 induction (OHT).

Figure 2

(A) Control cells (DMSO) show characteristic epithelial features, including compact, cobblestone like morphologies with high E-cadherin at the cell edges and low vimentin expression. 24 h OHT treatment results in decreased E-cadherin at the edges. 48 h and 72 h OHT treatment result in vimentin and cytoplasmic features with increased area and elongation. Scale = 50 μm. (B) GMM phenotypic classification shows that 0 h and 24 h are primarily epithelial (green bars and %), with rapid transition to mesenchymal phenotype at 48 h and 72 h (red bars and %), relative to the complete population (black line). N indicates the number of individual cells pooled per condition.