Table 1.
List of antibodies used
| No. | Name | Source | Phosphorylation sites | Concentration | Catalog no. | Manufacturer |
|---|---|---|---|---|---|---|
| 1 | p-JAK1 | Rabbit polyclonal | Tyr1022/1023 | 1:1000 | 3331 | Cell Signaling |
| 2 | JAK1 | Rabbit monoclonal | 1:1000 | 3344 | Cell Signaling | |
| 3 | p-SRC 416 | Rabbit polyclonal | Tyr416 | 1:1000 | 2101 | Cell Signaling |
| 4 | SRC | Rabbit monoclonal | 1:1000 | 2109 | Cell Signaling | |
| 5 | p-β-Arrestin1 | Mouse monoclonal | Ser412 | 1:1000 | 2416 | Cell Signaling |
| 6 | p-EGFR | Goat polyclonal | Tyr1173 | 1:500 | sc-12351 | Santa Cruz |
| 7 | EGFR | Rabbit polyclonal | 1:500 | sc-03 | Santa Cruz | |
| 8 | RAS | Rabbit polyclonal | 1:1000 | 3965 | Cell Signaling | |
| 9 | p-c-RAF | Rabbit monoclonal | Ser338 | 1:1000 | 9427 | Cell Signaling |
| 10 | c-RAF | Rabbit polyclonal | 1:1000 | 9422 | Cell Signaling | |
| 11 | p-ERK1/2 | Rabbit Polyclonal | Thr202/Tyr204 | 1:1000 | 9101 | Cell Signaling |
| 12 | ERK1/2 | Rabbit polyclonal | 1:1000 | 9102 | Cell Signaling | |
| 13 | p-AKT | Rabbit polyclonal | Ser473 | 1:1000 | 9271 | Cell Signaling |
| 14 | AKT | Rabbit polyclonal | 1:1000 | 9272 | Cell Signaling | |
| 15 | p-JNK | Rabbit polyclonal | Thr183/Tyr185 | 1:1000 | 9251 | Cell Signaling |
| 16 | JNK | Rabbit monoclonal | 1:1000 | 9258 | Cell Signaling | |
| 17 | p-P38 | Rabbit polyclonal | Thr180/Tyr182 | 1:1000 | 9211 | Cell Signaling |
| 18 | P38 | Rabbit polyclonal | 1:1000 | 9251 | Cell Signaling | |
| 19 | EGR1 | Rabbit polyclonal | 1:1000 | 4152 | Cell Signaling | |
| 20 | SP1 | Rabbit polyclonal | 1:500 | sc-59 | Santa Cruz | |
| 21 | ETS-1/2 | Rabbit polyclonal | 1:500 | sc-112 | Santa Cruz | |
| 22 | p-ELK-1 | Rabbit polyclonal | Ser383 | 1:1000 | 9181 | Cell Signaling |
| 23 | ELK-1 | Rabbit polyclonal | 1:1000 | 9182 | Cell Signaling | |
| 24 | SP3 | Rabbit polyclonal | 1:500 | sc-644 | Santa Cruz | |
| 25 | β-Actin | Mouse monoclonal | 1:8000 | A2228 | Sigma-Aldrich | |
| 26 | p-Serine | Rabbit polyclonal | Ser | 1:1000 | 9601 | Cell Signaling |
| 27 | p-Threonine | Mouse monoclonal | Thr | 1:1000 | 9386 | Cell Signaling |
| 28 | p-Tyrosine | Mouse monoclonal | Tyr | 1:2000 | 9411 | Cell Signaling |
| 29 | PGT | Rabbit polyclonal | 1:1000 | 160200 | Cayman Chemicals |