Fig. 2.

Pax6 binds to the A1-box of the rat insulin II promoter. A, For EMSA, nuclear extracts (ne) obtained from INS-1 cells were incubated with the double-stranded radiolabeled (superscript x) oligonucleotide RINSI-PISCES. The DNA-Pax6 complex (lane 2) was supershifted by a monoclonal antibody directed against Pax6 (lane 3). As a competitor, a 100-fold excess of unlabeled RINSI-PISCES was used (lane 4). B, Double-stranded radiolabeled oligonucleotide RINSII-A1 or C, RINSII-A3/A4 was incubated with 4 μl in vitro translation product Pax6. The DNA-Pax6 complex (lane 2) was supershifted by a monoclonal antibody directed against Pax6 (lane 3). Asterisks indicate unspecific signals.