Fig. 8.

Targeting of AC6Pal3 by decoy oligonucleotide abrogates the potentiation of PACAP-induced renin mRNA expression by rosiglitazone. Calu-6 cells were incubated for 16 h with 25 μm double-stranded decoy oligonucleotide containing AC6Pal3 or double-stranded scrambled AC6Pal3 oligonucleotide, which served as control. Renin (A), AC6 (B), or FABP-4 (C), together with β-actin (internal control) mRNA levels, were quantified by real-time PCR. Rosiglitazone was applied at 500 nm and PACAP at 300 nm. Rosi, Rosiglitazone. The data shown are means ± sem; *, P < 0.05 relative to the corresponding control; #, P < 0.05 relative to scrambled-PACAP.