Table 3.
Primers used in real-time PCR
| Gene | Sense primer | Antisense primer | Amplicon (bp) |
|---|---|---|---|
| hRen | atgaagggggtgtctgtggggtc | ggatatccatggcgtggatggcc | 304 |
| Human β-actin | cttctacaatgagctgcgtgtgg | gaggatcttcatgaggtagtcag | 313 |
| Human AC1 | ggcgagatgttgacatac | ctaagcctccttcccagctg | 223 |
| Human AC3 | gaagagagatgaggagctg | gccattggtgttgacatc | 262 |
| Human AC6 | cacatcagcagcatcgg | cagtccagcccatcaaaggtctc | 164 |
| Human/mouse AC7 | gggcctgtgattgctggagtg | cggtaacctggattttccc | 121 |
| Human AC8 | cagaatactctggctgccc | cttgtgctccaggctggagtc | 204 |
| Human AC9 | ggaacatcatcccctacc | catctccttggcgaactcg | 338 |
| Mouse AC5 | atgaagctgatggac | ctggccacattcactgtattgc | 155 |
| Mouse AC6 | cacatcagcagcatcgg | cagtccagcccatcaaaggtctc | 164 |
| Mouse Gsα | aaccgcctgcaggaggctc | caggagtggtgtagcgagc | 175 |
| Mouse β-actin | ccgccctaggcaccagggtg | ggctggggtgttgaaggtctcaaa | 285 |
The sequences are in 5′ to 3′ orientation.