Abstract
The solubility of cellulase extracted from the abscission zones of citrus leaf explants (Citrus sinensis L. Osbeck) in sodium phosphate buffer depends on the pH of the extracting solution and, to a lesser extent, on the ionic strength. By increasing molarity from 0.01 to 0.16, the solubility of cellulase increased from 51% to 89% at pH 6.1 and from 70% to 98% at pH 7. In all cases, residual cellulase was further extracted from the pellet by buffer containing 1 m NaCl. Most of the enzymic activity was found in tissues proximal to the separation line, and activity of the cellulase which was soluble in phosphate buffer was closely correlated with abscission at both pH values. When extraction of cellulase at pH 6.1 with phosphate buffer was followed by a reextraction of the pellet with buffer containing 1 m NaCl, the activity of the cellulase soluble in the fortified buffer was also correlated with abscission. Pretreatment of explants with ethylene increased the solubility of cellulase in the phosphate buffer regardless of the pH used at the first extraction.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abeles F. B. Abscission: role of cellulase. Plant Physiol. 1969 Mar;44(3):447–452. doi: 10.1104/pp.44.3.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Abeles F. B., Craker L. E., Leather G. R. Abscission: the phytogerontological effects of ethylene. Plant Physiol. 1971 Jan;47(1):7–9. doi: 10.1104/pp.47.1.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lewis L. N., Varner J. E. Synthesis of Cellulase during Abscission of Phaseolus vulgaris Leaf Explants. Plant Physiol. 1970 Aug;46(2):194–199. doi: 10.1104/pp.46.2.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]
- Rasmussen G. K. Changes in cellulase and pectinase activities in fruit tissues and separation zones of citrus treated with cycloheximide. Plant Physiol. 1973 Apr;51(4):626–628. doi: 10.1104/pp.51.4.626. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ratner A., Goren R., Monselise S. P. Activity of pectin esterase and cellulase in the abscission zone of citrus leaf explants. Plant Physiol. 1969 Dec;44(12):1717–1723. doi: 10.1104/pp.44.12.1717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reid P. D., Strong H. G. Cellulase and Abscission in the Red Kidney Bean (Phaseolus vulgaris). Plant Physiol. 1974 May;53(5):732–737. doi: 10.1104/pp.53.5.732. [DOI] [PMC free article] [PubMed] [Google Scholar]