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. 2010 Oct;24(10):1978–1987. doi: 10.1210/me.2010-0054

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Fig. 2. — Serotonergic agents affect adipocyte differentiation. A, Stable cell lines were generated from TPH1^mut cells by transduction with Cre recombinase. The concentration of 5-HT in these stable cell lines was measured by ELISA. Values are the means ± se from at least three experiments. *, P < 0.05 vs. TPH1^mut cells. B, The efficiency of differentiation was measured using the accumulation of oil red O by densitometric analysis in wild-type 3T3-L1, TPH1^mut, and TPH1^mut treated with 5-HT (20 μm). *, P < 0.05. C, Photographs show oil red O staining of differentiated adipocytes at various concentrations of 5-HT in 3T3-L1 cells and SVF. D and E, The efficiency of differentiation was measured using the accumulation of oil red O by densitometric analysis. Effects of 5-HT or PCPA on adipocyte differentiation in 3T3-L1 cells (D) and SVF (E). The data shown are means ± se from six experiments.*, P < 0.05; **, P < 0.01 vs. control cells.