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. 2010 Oct;24(10):1988–1997. doi: 10.1210/me.2010-0115

Fig. 1.

Fig. 1.

Glucose-dependent modulation of GK binding to the GRP. The interaction strength of the fusion proteins DNA-BD-GRP and AD-GK was measured in a fluorescence-based mammalian two-hybrid assay. DNA-BD and AD-GK (white bars) or DNA-BD-GRP and AD (dashed bars) or DNA-BD-GRP and AD-GK (black bars) were coexpressed in COS cells. The cells were preincubated in the presence of 5 mmol/liter or 25 mmol/liter glucose. Thereafter, the fluorescence intensities of ECFP and EYFP were determined. Data are expressed as means ± sem of three to five individual experiments with a total of 142–266 nuclei analyzed. **, P < 0.01; ***, P < 0.001 compared with negative controls; ##, P < 0.01 compared with cells incubated with 5 mmol/liter glucose (ANOVA/Bonferroni’s test).