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. 2010 Oct;24(10):1988–1997. doi: 10.1210/me.2010-0115

Fig. 2.

Fig. 2.

Glucose promoted binding of islet PFK-2/FBPase-2 to GK. The interaction strength of the fusion proteins DNA-BD-PFK-2/FBPase-2 or DNA-BD-PFK-2/FBPase-2-Mut and AD-GK was measured in a fluorescence-based mammalian two-hybrid assay in insulin-secreting MIN6 cells. A, DNA-BD-PFK-2/FBPase-2 and AD (white bars) or DNA-BD-PFK-2/FBPase-2 and AD-GK (black bars) were coexpressed in MIN6 cells and preincubated with 3 mmol/liter or 25 mmol/liter glucose. Thereafter, the fluorescence intensities for ECFP and EYFP were determined. Data are expressed as means ± sem of three individual experiments with a total of 60–70 nuclei analyzed. **, P < 0.01 compared with negative control (ANOVA/Bonferroni’s test). B, DNA-BD-PFK-2/FBPase-2 and AD-GK (black bars) or DNA-BD-PFK-2/FBPase-2-Mut and AD-GK (gray bars) were coexpressed in MIN6 cells and preincubated with 3 mmol/liter or 25 mmol/liter glucose. Thereafter, the fluorescence intensities for ECFP and EYFP were determined. Data are expressed as means ± sem of three individual experiments with a total of 44–152 nuclei analyzed. *, P < 0.05 compared with cells incubated with 3 mmol/liter glucose; #, P < 0.05 compared with DNA-BD-PFK-2/FBPase-2 wild type (ANOVA/Bonferroni’s test).