Table 2.
Expression of pituitary markers in adenomas from p27Kip1−/− and Tg-PCE;p27Kip1−/− pituitaries
| Wt pituitary | p27Kip1 −/− | Adenomas | Tg-PCE;p27Kip1−/− | Adenomas | ||
|---|---|---|---|---|---|---|
| Staining | IL | AL | IL | AL | IL | AL |
| Pitx1 | + | + | + | + | + | + |
| Brg1 | + | + | + | + | + | + |
| Tpit | + | + (5–10%) | + | − | + | One cell cluster (1/4) |
| SF-1 | − | + (5–10%) | − | Dispersed cells ∼ 5% | − | Dispersed cells ∼ 5% (1/4) |
| Pit-1 | − | + (>60%) | − | − | − | − |
| CyclinE | − | − | − | − | + | + (90%) |
| Sox2 | Around lumen | − | − | − | − | |
| Sox9 | Around lumen | − | − | − | − | |
| n = 12 | n = 12 | n = 9 | n = 1 | n = 10 | n = 4 |
The number of pituitary adenomas analyzed for marker expression by immunohistochemistry are indicated at bottom and correspond to adenomas described in Table 1. IL adenomas were positive for Tpit in both genotypes and negative for all other lineage markers. The single AL adenoma from p27Kip1−/− pituitary stained positively for SF-1 (∼5% cells). One Tg-PCE;p27Kip1−/− pituitary AL adenoma exhibited a similar SF-1-positive adenoma, and another AL adenoma contained a cluster of Tpit-positive cells and a mass of undifferentiated cells. All other AL adenomas cells appeared undifferentiated. +, >95% positive cells; –, no positive cells.