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Methylation analysis of the LAMA5 region by bisulfite treatment and direct sequencing. The peak heights in the sequencing chromatogram were visually evaluated to quantify the respective amount of methylated C vs. unmethylated C at each CpG position. A, Average values of methylation at each of the six analyzed CpGs. B, Ratios of methylation between ectopic and eutopic endometrium at each CpG position. *, P < 0.05; **, P < 0.01; ****, P < 0.001.
