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. 2010 Aug;24(8):1569–1580. doi: 10.1210/me.2010-0034

Fig. 9.

Fig. 9.

Enhanced expression of E-cadherin diminishes EGF-induced cell invasion. A, SKOV3 cells were transfected for 48 h with control vector (pIRES) or vector containing full-length E-cadherin cDNA (pIRES-EC). After transfection, cells were treated with 100 ng/ml EGF for 24 h, and E-cadherin protein levels were analyzed by Western blot. B, After 48 h of transfection, cells were treated with 100 ng/ml EGF, seeded into Matrigel-coated transwell inserts, and cultured for 24 h. Noninvading cells were wiped from the upper side of the filter, and the nuclei of invading cells were stained with Hoechst 33258. Top panel shows representative photos of the invasion assay. Scale bar, 200 μm. Bottom panel shows summarized quantitative results, which are expressed as mean ± sem of at least three independent experiments. *, P < 0.05 compared with control (Ctrl); #, P < 0.05 compared with EGF. †, P < 0.05 compared with pIRES-EC Ctrl.