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. 2010 Jul;24(7):1338–1348. doi: 10.1210/me.2009-0402

Fig. 5.

Fig. 5.

Effect of AR deficiency on Akt phosphorylation, Tfam expression, and interaction of phosphatidylinositol 3-kinase regulatory subunit α in H9c2 Cells. A, Akt phosporylation after AR siRNA transfection with or without Dox treatment in H9c2 cells. Representative blots of phosphorylated Akt and total Akt are presented. B, Tfam expression after AR siRNA transfection with or without Dox treatment in H9c2 cells. Representative blots of Tfam and β-actin are presented. C, Action of androgen on AR interaction with p85α in H9c2 cells. Left panel, Representative blots of p85α and AR expression. Right panel, A result of densitometric analysis of p85α expression corrected by AR. IP, Immunoprecipitation: WB, Western blot analysis. Values are expressed as means ± sem. *, P < 0.05 vs. with control, n = 4 in each group.