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. 2010 Jul;24(7):1338–1348. doi: 10.1210/me.2009-0402

Fig. 6.

Fig. 6.

Effect of androgen action on cell viability and apoptosis with or without Dox in H9c2 Cells. H9c2 cells were pretreated with or without flutamide or LY294002 for 1 h before testosterone (T) administration. After 24 h, cells were coincubated with Dox for 24 h and then analyzed. A, Cell viability after Dox treatment for 24 h in H9c2 cells. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt assay. Values are relative change to control value and expressed as means ± sem. *, P < 0.05; **, P < 0.01, n = 6 in each group. B, Apoptotic cells after Dox treatment of H9c2 cells. Upper panel, Representative photomicrographs of TUNEL-positive cells. The nuclei of apoptotic cells were determined by TUNEL staining (green), and total nuclei were demonstrated by DAPI staining (blue). Lower panel, The percentage of TUNEL-positive cells in three random fields. Values are relative change to control value and expressed as means ± sem. *, P < 0.05; **, P < 0.01, n = 4 in each group.