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. 2010 Jun;24(6):1267–1273. doi: 10.1210/me.2010-0056

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Fig. 4. — Effects of siRNA-mediated silencing of Runx2 on thyroid-specific gene expression in thyrocytes. A, mRNA (1 μg) from FRTL-5 cells stably transfected with pSilencer NC (lane 1) or with pSilencer-siRunx2 (lane 2) was transferred to cellulose acetate membranes and hybridized with [³²P]cDNAs for Runx-2, TSHR, NIS, Tg, TPO, and GAPDH. B, Tg and NIS mRNA levels in wild-type (lane 1) and heterozygous (lane 2) mice. mRNA (1 μg) from thyroid glands of wild-type and Runx2^+/− mice was transferred to the membrane, and levels of Tg and NIS mRNA were also determined.