Fig. 4.

GSK3α and GSK3β interact with Bim after exposure of thymocytes (A) and 2B4 T cells (B) to Dex. Thymocytes (A) and 2B4 T cells (B) were either untreated or treated with 100 nm Dex for the indicated time intervals. Cell lysates were immunoprecipitated with antibodies to Bim and processed for Western blot analysis using nonreduced protein sample buffer. Coimmunoprecipitated GSK3α and GSK3β were detected by Western blotting as described in Fig. 3B.