Table 1.
Gene or promoter region | Primer sequences (5′–3′) | Product length (bp) | Annealing temperature (C) |
---|---|---|---|
GAPDH | |||
Forward | GGGGAGCCAAAAGGGTCATCATCT | 457 | 58 |
Reverse | GACGCCTGCTTCACCACCTTCTTG | ||
Aromatase | |||
Forward | GAATATTGGAAGGATGCACAGACT | 343 | 58 |
Reverse | GGGTAAAGATCATTTCCAGCATGT | ||
ERRα | |||
Forward | AAAGTGCTGGCCCATTTCTAT | 100 | 60 |
Reverse | CCTTGCCTCAGTCCATCAT | ||
EP1 | |||
Forward | CGCTATGAGCTGCAGTACCC | 508 | 58 |
Reverse | CCAGGATCTGGTTCCAGGAG | ||
EP2 | |||
Forward | CAACCTCATCCGCATGCAC | 421 | 58 |
Reverse | CTCAAAGGTCAGCCTG | ||
EP3 | |||
Forward | ACCCGCCTCAACCACTCCTACACA | 410 | 58 |
Reverse | ATGGCGCTGGCGATGAACAAC | ||
EP4 | |||
Forward | CCTCCTGAGAAAGACAGTGCT | 367 | 58 |
Reverse | AAGACACTCTCTGAGTCCT | ||
PI.3/II | |||
Forward | CCCACTCAAGGGCAAGAT | 118 | 58 |
Reverse | CAAGGAAGCCCAAGAAAG |