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. 2010 Jun;24(6):1175–1186. doi: 10.1210/me.2009-0470

Table 1.

Primers used for PCR

Gene or promoter region Primer sequences (5′–3′) Product length (bp) Annealing temperature (C)
GAPDH
    Forward GGGGAGCCAAAAGGGTCATCATCT 457 58
    Reverse GACGCCTGCTTCACCACCTTCTTG
Aromatase
    Forward GAATATTGGAAGGATGCACAGACT 343 58
    Reverse GGGTAAAGATCATTTCCAGCATGT
ERRα
    Forward AAAGTGCTGGCCCATTTCTAT 100 60
    Reverse CCTTGCCTCAGTCCATCAT
EP1
    Forward CGCTATGAGCTGCAGTACCC 508 58
    Reverse CCAGGATCTGGTTCCAGGAG
EP2
    Forward CAACCTCATCCGCATGCAC 421 58
    Reverse CTCAAAGGTCAGCCTG
EP3
    Forward ACCCGCCTCAACCACTCCTACACA 410 58
    Reverse ATGGCGCTGGCGATGAACAAC
EP4
    Forward CCTCCTGAGAAAGACAGTGCT 367 58
    Reverse AAGACACTCTCTGAGTCCT
PI.3/II
    Forward CCCACTCAAGGGCAAGAT 118 58
    Reverse CAAGGAAGCCCAAGAAAG