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. 2017 May 4;12(5):e0175543. doi: 10.1371/journal.pone.0175543

Table 2. List of primers used in this study.

Primer Primer direction Primer sequence Position Restriction enzyme
HBVs Forward CCGGAAAGCTTATGCTCTTCTTTTTCACCTAATCATC 1821–1843 BspQI (Underlined Complete restriction site)
HBVas Reverse CCGGAGAGCTCATGCTCTTCAAAAAGTTGCATGGTGCTGGTG 1825–1804 BspQI (Underlined Complete restriction site)
HBV1 Forward GTCAACGACCGACCTTGAGGC 1684–1704 -
HBV2 Reverse TGAACAGACCAATTTATGCCTACAGCCTCC 1805–1776 -
HBV3 Forward ATGGAGACCACCGTGAACGC 1608–1627 -
HBV4 Reverse AATTCTTTATAAGGATCAATGTCCAGGCCC_ 1926–1897 NIaIV (Underlined Partial restriction site)
HBV5 Forward CTGTGCCAAGTGTTTGCTGA 1171–1190 -
HBV6 Forward CTGCTGGTGGCTCCAGTTC 57–75 -
HBV7 Reverse AACGCCGCAGACACATCCA 391–373 -
HBV8 Forward ATTTTACGGGACTCTATTCCTC 2479–2500 -
HBV9 Reverse GGGACTCAAGATGYTGYACAG 789–769 -