Table 2. List of primers used in this study.
Primer | Primer direction | Primer sequence | Position | Restriction enzyme |
---|---|---|---|---|
HBVs | Forward | CCGGAAAGCTTATGCTCTTCTTTTTCACCTAATCATC | 1821–1843 | BspQI (Underlined Complete restriction site) |
HBVas | Reverse | CCGGAGAGCTCATGCTCTTCAAAAAGTTGCATGGTGCTGGTG | 1825–1804 | BspQI (Underlined Complete restriction site) |
HBV1 | Forward | GTCAACGACCGACCTTGAGGC | 1684–1704 | - |
HBV2 | Reverse | TGAACAGACCAATTTATGCCTACAGCCTCC | 1805–1776 | - |
HBV3 | Forward | ATGGAGACCACCGTGAACGC | 1608–1627 | - |
HBV4 | Reverse | AATTCTTTATAAGGATCAATGTCCAGGCCC_ | 1926–1897 | NIaIV (Underlined Partial restriction site) |
HBV5 | Forward | CTGTGCCAAGTGTTTGCTGA | 1171–1190 | - |
HBV6 | Forward | CTGCTGGTGGCTCCAGTTC | 57–75 | - |
HBV7 | Reverse | AACGCCGCAGACACATCCA | 391–373 | - |
HBV8 | Forward | ATTTTACGGGACTCTATTCCTC | 2479–2500 | - |
HBV9 | Reverse | GGGACTCAAGATGYTGYACAG | 789–769 | - |