Table 1.
Primer sequences
| Gene name | Primer sequences (5′–3′) | Product size (bp) | Number of cycles | Annealing temperature (°C) |
|---|---|---|---|---|
| ErbB2-HER2 | ||||
| Forward | CCATAACACCCACCTCTGCT | 194 | 20 | 58–68 |
| Reverse | ACTGGCTGCAGTTGACACAC | |||
| EGFR (80) | ||||
| Forward | ATGTCCGGGAACACAAAGAC | 351 | 40 | 56 |
| Reverse | TTCCGTCATATGGCTTGGAT | |||
| cPLA2 | ||||
| Forward | ACGTTTCAGAGCTGATGTTT | 352 | 30 | 52–62 |
| Reverse | CTTCCAGCATCTTCATTTTC | |||
| COX-2 | ||||
| Forward | TGAAACCCACTCCAAACACA | 187 | 40 | 58–63 |
| Reverse | GAGAAGGCTTCCCAGCTTTT | |||
| GAPDH | ||||
| Forward | GTCATCATCTCTGCCCCCTCTGC | 444 | 14 | 52 |
| Reverse | CGACGGCTGCTTCACCACCTTCT |