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ChIP demonstrated that cortisol (1 μm) stimulated the binding of GR to the GRE in the cPLA2α promoter as revealed by gel electrophoresis of the PCR products amplified from the DNA fragments precipitated by GR antibody in human amnion fibroblasts. The top panel is a representative gel of three individual experiments; bottom panel illustrates the positions and sequences of the primers used for PCR.
