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Stimulation of cPLA2α mRNA (A, n = 3) and protein (B) expression by cortisol (1 μm) was attenuated by transfection of a vector expressing dn-CREB-1 protein in human amnion fibroblasts. The top panel of B is the representative immunoblot; bottom panel shows the mean data of three individual experiments. *, P < 0.05 vs. the fold induction in cells transfected with negative control vector (NC).
