Fig. 8.
A, Representative immunoblot showing GR in the protein complex immunoprecipitated (IP) by GR antibody (Ab) or CREB-1 antibody in human amnion fibroblasts stimulated by cortisol (1 μm). IgG(H), IgG heavy chain; IgG(L), IgG light chain. B, ChIP demonstrated that cortisol (1 μm) stimulated the binding of CREB-1 to the GRE in the cPLA2α promoter as revealed by gel electrophoresis of the PCR products amplified from the DNA fragments precipitated by CREB-1 antibody in human amnion fibroblasts. The top panel is the representative gel image of three individual experiments; bottom panel illustrates the positions and sequences of the primers used for PCR.