Table 2. Performance characteristics of Multiplex HIV-1 incidence kit.
| Biomarker | Cutoff Value | Non-Parametric Survival (95% CL) | Logit Random Intercept (95% CL) | FR/Total LT | Crude FRR (95% CL) |
Averaged FRRb (95% CL) |
|---|---|---|---|---|---|---|
| gp120-n | 2.8 | 278.2 (228.8, 327.6) | 263.6 (218.0, 316.5) | 3/334 | 0.9 (0.2, 2.6) | 1.2 (0.2, 7.2) |
| gp160-n | 2.5 | 206.5 (165.6, 247.5) | 195.5 (160.2, 235.4) | 0/334 | 0.0 (0.0, 1.1) | 0.0 (0.0, 5.3) |
| Mean Normalized MFI | 2.6 | 228.1 (186.7, 269.5) | 219.3 (181.5, 261.1) | 0/334 | 0.0 (0.0, 1.1) | 0.0 (0.0, 5.3) |
| gp120-a | 20 | 328.5 (278.7, 378.4) | 325.1 (277.0, 375.3) | 13/334 | 3.9 (2.1, 6.6) | 4.6 (2.0, 11.8) |
| gp160-a | 35 | 272.4 (238.4, 306.3) | 262.0 (226.8, 301.2) | 1/334 | 0.3 (0.0, 1.7) | 0.2 (0.0, 5.6) |
| gp41-a | 65 | 281.7 (242.3, 321.2) | 284.9 (247.7, 326.1) | 2/334 | 0.6 (0.1, 2.1) | 0.4 (0.0, 5.9) |
| Mean Avidity Index | 40 | 271.9 (233.7, 310.1) | 271.3 (235.3, 312.3) | 0/334 | 0.0 (0.0, 1.1) | 0.0 (0.0, 5.3) |
| Multi-Analyte Algorithma | 3/5 ≥ Respective Cutoff is Non-Recent | 250.7 (211.1, 290.3) | 243.8 (206.7, 287.8) | 0/334 | 0.0 (0.0, 1.1) | 0.0 (0.0, 5.3) |
| Principal Component | 0 | 225.3 (183.7, 266.9) | 223.4 (188.6, 264.0) | 0/334 | 0.0 (0.0, 1.1) | 0.0 (0.0, 5.3) |
| Standardized Score | 0 | 219.4 (179.1, 259.6) | 221.4 (187.3, 260.8) | 0/334 | 0.0 (0.0, 1.1) | 0.0 (0.0, 5.3) |
aCutoff values: 120a = 20, 160a = 35, 41a = 65, 120n = 2.8, 160n = 2.5.
bN = 296 MSM cohort + 38 longitudinal seroconverters; averaged across 4 CD4 strata (assumes Bio-plex observations in 800+ stratum).