Fig. 7.

Chronic inhibition of the mTORC1/S6K1 pathway reduces insulin-induced glucose uptake in 3T3-L1 adipocytes. After siRNA or chronic rapamycin treatments, cells were serum deprived and stimulated for 45 min with 10 nm insulin before glucose uptake was measured using (A) 2-D-deoxyglucose uptake (n = 10), (B) 3-O-methylglucose uptake (n = 3), and (C) 2-D-deoxyglucose in the presence of the GLUT4 inhibitor indinavir (n = 4). Glucose uptake inhibited by indinavir therefore represents GLUT4-mediated glucose uptake. Glucose uptake was normalized for protein content. Results are shown as means ± se. Groups with different letters are significantly different (P < 0.05).