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Chronic mTORC1/S6K1 pathway disruption slightly affects protein and lipid contents in 3T3-L1 adipocytes. A, Protein content was measured by colorimetry (n = 7). B, Lipid accumulation in 3T3-L1 adipocytes was measured by Oil-Red-O staining (n = 4). C, PPARγ mRNA expression was measured by quantitative real-time PCR (n = 3). Results are shown as means ± se. Groups with different letters are significantly different (P < 0.05).
