High-Throughput Sequencing of RNA Isolated by Cross- Linking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs

. Author manuscript; available in PMC: 2017 May 4.

Published in final edited form as: Methods Mol Biol. 2014;1125:187–208. doi: 10.1007/978-1-62703-971-0_17


1 mL	10× TBE

2 mL	30 % acrylamide and bis-acrylamide solution, 37.5:1 ratio

120 μL	10 % ammonium persulfate

20 μL	TEMED

6.88 mL	Water