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. 2017 May 4;12(5):e0177112. doi: 10.1371/journal.pone.0177112

Fig 6. Real-time PCR analysis of Bacillus atrophaeus DNA in soil samples.

Fig 6

Serial dilutions (from 2.4% to 0.081%) of the DNA extracts for control soil samples and soil samples inoculated with 10, 103, or 105 Bacillus atrophaeus cfu were analysed using a TaqMan-PCR assay. The figure shows representative amplification plots for PCR triplicates. Control soil samples and soil samples inoculated with 10 Bacillus atrophaeus cfu never yielded a PCR signal. The blue horizontal line corresponds to the threshold for the determination of the CT.