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Apoptosis rate of cells in each group estimated by flow cytometry. (A–E) Apoptotic cell distribution of TPC-1 cells in groups of control (A), NC (B), miRNA-221 inhibitors (C), siTIMP3+ miRNA-221 inhibitors (D), and Lenti-TIMP3 (E). (F) Relative apoptosis rate of cells in each group. Data were represented as mean ± SD for 3 independent experiments. *P < 0.05 vs control group, #P < 0.05 vs NC group, ○P < 0.05 vs inhibitors group, □P < 0.05 vs siRNA+inhibitors group.
