FIGURE 3.

Transmission electron microscopy tissue localization of Gd-containing spots in the region of the lateral (dentate) cerebellar nuclei in the brain after the repeated high-dose application of gadodiamide (20 × 2.5 mmol Gd/kg body weight). TEM evaluation showed several positive signals: (A) 1 single focus (original magnification, ×18,900) and (B) several electron-dense signals occurring as multiple roundish nodules with variable diameter (original magnification, ×18,800). The location indicates intracellular deposition within endothelial cell of blood vessels; 1 signal appeared to be adjacent to an endothelial cell on the adluminal side. The EDX analysis showed, compared with the control area (C), Gd-positive signals (D, arrows). No positive signals could be detected in neurons or in the neuropil.