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. 2017 May 4;12(5):e0176994. doi: 10.1371/journal.pone.0176994

Fig 5. The expression of CXCL11 and CCL5 is positively correlated with IFNγ expression in lesional psoriatic skin.

Fig 5

(A) Chemotaxis assays were performed using a 24-well plate-based assay. PBMCs were isolated from blood taken from psoriatic patients and loaded on the upper chamber containing a 5-μm pore-size filter. Wells (lower chamber) were added vehicle or 10 nM recombinant CXCL11 or CCL20 and incubated for 20 hours. Cells migrating to the lower chamber were analyzed by flow cytometry (n = 9). Results are expressed as mean ± standard deviation. *P < 0.01. (B-E) RNA from punch biopsies taken from 16 psoriatic patients was isolated and the mRNA expression of CXCL11, CCL5, IFNγ, and IL-17A analyzed by qPCR. The correlation between the expression levels of these cytokines was analyzed as indicated in the figure.