Fig 2. Identification of Ten-2 splice variant.
A, Position of PCR primers (arrowheads), expected amplification product (solid line), and 27 bp insert within the Ten-2 mRNA. Vertical lines denote predicted splice sites and rhombs mark the position of extracellular EGF-repeats. B, Ten-2 RT-PCR amplification products obtained in breast and ovarian cancer cells using primers outlined in A. The position of bands (400 and 427 bp) is marked at the right. C, Scheme showing site of alternative splicing, and sequence of 27 bp insert with predicted amino-acids based on predicted, in-frame ORF.