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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Sci Signal. 2016 Aug 16;9(441):ra82. doi: 10.1126/scisignal.aae0453

Fig. 5. The p110β-dependent generation of PIP3 in BMNs in response to adhesion to poly-RGD+ requires both an intact RBD and binding to Gβγ proteins.

Fig. 5

BMNs from WT, β-RBD, β-Gβγ, and β-KO mice were preincubated with 40 nM TGX221 or vehicle control before being added to glass coverslips coated with poly-RGD+ or were left in suspension. Where indicated, WT BMNs were additionally preincubated with 100 nM wortmannin (wort). Cells were allowed to adhere and spread for 10 min at 37°C and then harvested for PIP3 measurement by MS as described earlier. Data are means ± SEM of PIP3/PI ratios from three experiments (except for data from wortmannin-treated cells, for which n = 2), each performed in duplicate. N.D., not determined. *P < 0.05 by one-sample t test with Holm-Sidak correction for multiple comparisons.