Figure 3.
Diffusion coefficient calculated by fluorescence recovery after photobleaching (FRAP) for the free AF488 (blue) and AF488-loaded polymeric nanoparticles (red) in simulated mucus at different pH’s. a) After incubation for 1.3 h at RT; b) after incubation for 13 h at 4°C. (c) Relative diffusivity of AF488 with particles vs. free AF488 for 1.3 h (blue) and 13 h (red). d) Viability of MDA-MB-231 cells after 24 (blue) and 48 h (red) incubation with indicated concentration of polymer particles.
