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. 2017 Apr 20;13(4):e1006736. doi: 10.1371/journal.pgen.1006736

Fig 1. Construct of the strain library for screening long-distance regulation.

Fig 1

A) Reporter gene cassette containing MET3pr driving GFP. Tm: Terminator. HL and HR: mTn homolog sequences. MET3pr is located in the middle of the cassette so that it is far from the local sequences at the integration sites. B) Procedure of library construction. MET3pr-GFP was inserted into the mTn site, and MET3pr-mCherry was then inserted into the CDC20 locus. Library strains were grown under inductive conditions (no methionine), and the steady-state level of GFP and mCherry were measured in single cells using flow cytometry. Typical processed FACS data (yellow) are shown along with background control (black) and GFP only (green) / mCherry only (red) controls.