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Functional validation of significant protein signatures by siRNA-mediated knockdown. Three siRNAs per gene (Thermo Fisher) were used to transfect A549 cells for 48 h. Then, cells were infected with Pan or Mal virus (MOI 0.02) for 39 and 48 h, respectively. Supernatants were titrated by infection of MDCKII cells for 7 h and NP staining. Normalized values were displayed as Z-score in relation to NP (positive control) and Allstars siRNA (negative control); n = 3. Cut-off: ± 1.5.
