Table I. Distribution of O-glycan features on mucins from gastric adenocarcinoma tumor (Tumor), normal mucosa of tumor-adjacent tissue (Norm.tum.adj) and normal tissue (Normal). The relative amounts of the different glycan features are given in percentage (%) in the relation to the total sum of integrated peak areas in the LC-MS chromatograms.
| Samplesa | Core 2b | Core 1 | I antigen | Blood group | H type | A type | B type | AB type | Lea/x | sLea/x | Leb/y | Fucosylation | α1,4-GlcNAc | LacdiNAc | sTn | Sialylation | Sulfation | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tumor | P1TS | 81.1 | 17.9 | 1.1 | B | 89.2 | − | 0.2 | − | 0.9 | 0.6 | 0.1 | 90.6 | 4.0 | 6.3 | 0.7 | 8.9 | 0.8 |
| P1TI | 63.7 | 26.8 | 0.3 | B | 29.3 | − | 8.9 | − | 10.0 | 7.9 | 0.6 | 69.4 | 5.8 | 0.7 | 3.3 | 22.8 | 1.1 | |
| P3TS | 62.7 | 35.1 | 2.1 | H | 31.2 | − | − | − | 0.7 | − | − | 31.9 | 6.5 | − | − | 21.3 | 7.1 | |
| P2TS | 62.9 | 22.1 | 2.8 | A | 17.2 | 3.3 | − | − | 2.7 | 2.0 | − | 24.3 | 8.3 | 0.9 | 10.6 | 59.8 | 15.7 | |
| P4TS | 63.8 | 22.8 | 4.7 | A | 42.5 | 4.5 | − | − | 4.1 | 1.7 | − | 47.0 | 18.5 | 1.2 | 11.3 | 24.7 | 21.1 | |
| P4TI | 85.9 | 12.7 | 1.4 | A | 56.1 | 20.2 | − | − | 0.7 | 0.4 | − | 83.6 | 7.3 | 5.8 | − | 4.2 | − | |
| Norm.tum.adj | P6TA-FS | 84.0 | 12.3 | 5.4 | A | 11.1 | 1.3 | − | − | 6.7 | 1.0 | − | 18.9 | 5.4 | 3.2 | 0.3 | 9.2 | 0.3 |
| P6TA-FG | 77.1 | 18.8 | 9.1 | A | 50.6 | 18.9 | − | − | 5.7 | − | 0.2 | 73.7 | 16.5 | 1.3 | 0.5 | 4.7 | 0.3 | |
| P6TA-AS | 80.0 | 16.7 | 1.3 | A | 69.5 | 16.4 | − | − | 1.0 | − | − | 86.0 | 5.5 | 2.4 | − | 6.8 | 1.3 | |
| P6TA-AG | 85.3 | 14.7 | 22.5 | A | 33.3 | 6.4 | − | − | 18.9 | 0.8 | − | 58.7 | 32.1 | − | − | 14.1 | − | |
| P5TA-AS | 62.3 | 23.4 | 0.8 | AB | 19.1 | 0.9 | 4.2 | 0.3 | 26.0 | 23.7 | 0.5 | 45.5 | 1.6 | 0.2 | 12.5 | 67.7 | 1.4 | |
| P5TA-AG | 68.8 | 20.9 | 5.5 | AB | 22.5 | 18.9 | 8.7 | 3.5 | − | − | − | 53.6 | 21.6 | 0.8 | − | − | − | |
| P7TA-AS | 68.8 | 13.2 | − | AB | 71.7 | − | − | − | − | − | − | 61.0 | − | − | − | 21.5 | 33.6 | |
| P7TA-AG | 83.4 | 17.0 | 1.9 | AB | 34.9 | 50.9 | 2.3 | 1.3 | 14.8 | − | 6.1 | 93.3 | 9.6 | 3.6 | 0.3 | 1.0 | 0.4 | |
| Normal | P8H | 78.0 | 19.1 | 4.3 | A | 50.4 | 7.9 | − | − | 6.0 | 0.3 | 0.9 | 62.2 | 3.6 | 2.1 | 0.9 | 9.5 | 0.3 |
| P9H | 93.2 | 5.4 | 4.5 | B | 64.8 | − | 15.8 | − | 7.0 | 0.6 | 0.5 | 88.8 | 8.0 | 8.0 | 1.3 | 7.1 | − | |
| P10H | 75.4 | 19.1 | 1.1 | AB | 46.9 | 0.8 | 1.2 | 0.6 | 15.5 | 13.1 | 1.4 | 68.5 | 3.9 | 2.5 | 2.2 | 37.6 | 1.1 | |
aTen Samples included four specimens (P1-P4) from gastric adenocarcinoma tumors (T), three (P5-P7) from normal mucosa of tumor-adjacent tissues (TA), and three (P8–10) from normal tissue (H). For mucins from tumors, they were divided into soluble (S) and insoluble mucins (I). Mucins from tumor-adjacent tissues were separated into fundus (F) versus pyloric antrum (A), surface (S) versus gland material (G) according to tissue location.
bIdentification of structural epitopes was based on knowledge of gastric glycan biosynthesis and assumptions made on linkage configuration and positions were summarized in material and methods.