Table II. Acetylation site stoichiometry at CobB-regulated sites. The table shows the number of acetylation sites and their median stoichiometry in the indicated experiments, classified according to increased acetylation in cobBΔ EP cells compared to WT EP cells. Sites with greater than 2-fold (CobB >2), 4-fold (CobB >4), and 8-fold (CobB >8) increased acetylation are indicated. WT = wild type, EP = exponential phase, SP = stationary phase, NAM = nicotinamide, and knock out (Δ) strains are as indicated.
All sites | CobB >2 | CobB >4 | CobB >8 | |
---|---|---|---|---|
WT EP | ||||
Number | 2243 | 357 | 167 | 87 |
Median stoichiometry | 0.042% | 0.018% | 0.009% | 0.006% |
cobBΔ EP | ||||
Number | 1663 | 285 | 138 | 68 |
Median stoichiometry | 0.223% | 0.206% | 0.192% | 0.168% |
WT SP | ||||
Number | 2243 | 357 | 167 | 87 |
Median stoichiometry | 0.054% | 0.078% | 0.094% | 0.103% |
cobBΔ SP | ||||
Number | 1657 | 293 | 293 | 80 |
Median stoichiometry | 0.252% | 0.319% | 0.319% | 0.517% |
ptaΔ | ||||
Number | 1462 | 228 | 101 | 49 |
Median stoichiometry | 0.028% | 0.016% | 0.009% | 0.007% |
ptaΔ NAM | ||||
Number | 832 | 141 | 74 | 36 |
Median stoichiometry | 0.029% | 0.025% | 0.024% | 0.023% |
ackAΔ | ||||
Number | 1474 | 225 | 99 | 44 |
Median stoichiometry | 0.266% | 0.157% | 0.076% | 0.039% |
ackAΔ NAM | ||||
Number | 1594 | 282 | 139 | 73 |
Median stoichiometry | 0.360% | 0.447% | 0.459% | 0.465% |