Fig. 2.

1NM-PP1 causes off-target phosphorylation events. (a) In order to estimate the effect of 1NM-PP1 treatment alone a wild type (non-as) strain was treated with 10 μm 1NM-PP1 for 15 min and compared with DMSO control treatment. Significant changes were observed on ∼13% of detected phosphosites. (b) MotifX analysis revealed basophilic kinase motifs among the dephosphorylated sites in a 1NM-PP1 treated wt strain. NetworKIN analysis also suggested that ∼60% of dephosphorylated sites were predicted substrates of the basophilic kinase PKA (Tpk1, Tpk2, and Tpk3).